Verhoeff van Gieson
Classification: connective tissue stain
Mechanism of staining: ionic bonding/van de Waals forces for elastin fibers
Purpose: stain elastin
Control tissue: aorta, skin, lung
Well Stained Slide
Elastic fibers – blue-black to black
Nuclei – blue/grey/black
Collagen – red
Muscle – orange
RBCs, cytoplasm – yellow
Purpose of VVG: Demonstration of pathologic elastin fibers, atrophy of elastic fibers, arterioclerosis or other vascular disorders, breaking of elastic fibers due to ageing process and demonstration of elastic fibers in normal tissues (veins and arteries)
Proper preparation of Verhoeff’s Iron hematoxylin solution is critical for successful staining. Usually 20ml of alcoholic Hematoxylin is mixed with 8ml of Ferric chloride(10%) and 8ml of lugols Iodine. This compound should be prepared prior to use and the ingredients must be added in order because if iodine is added first, the hematoxylin immediately would be overoxidized and a substance without staining properties would be made.The counterstain (Van Gesson) is usually used with pH between 1.0 & 2.0 which increases the number of dye binding sites for collagen. When doing the dehydration, it should be done rapidly so that the picric acid will not be lost from cytoplasm.
There is no need to remove mercury pigments by a separate procedures because Iodine will automatically remove these pigments during the staining method.
Clean glassware and staining tray with 2% FeCl3
Officer B. HIML251 Lecture notes: Elastic Stains: Verhoeff’s van Gieson & Gomori’s Aldehyde Fuchsin, January 21, 2009